ABSTRACT
Chemical constituents were isolated from the fruiting bodies of Ganoderma australe by various column chromatographic techniques and HPLC method, and their chemical structures were identified through the combined analysis of physicochemical properties and spectral data. Meanwhile, their α-glucosidase inhibitory activity and anti-oxidative ability were evaluated. Seven compounds were isolated from G. australe and were identified as 6-methoxyl-cyclo-(Phe-Ile)(1), applanoxidic acid A methyl ester(2), ergosta-7,22 E-dien-3β-ol(3), cinnamic acid(4), 5α,8α-epidioxy-(20S,22E,24R)-ergosta-6,22-diene-3β-ol(5), 1-(3, 4-dihydroxyphenyl) ethanone(6), salicylic acid(7) and benzoic acid(8). Among the compounds, compound 1 was a new cyclic dipeptide. Compound 2 was a new lanosta natural product, and compounds 4, 6, 7 and 8 were obtained from G. australe for the first time. Moreover, compounds 4 and 8 exhibited α-glucosidase inhibitory activity with inhibition rates of 36.8% and 34.7%, and compounds 4, 7 and 8 had a certain activity in DPPH free radical scavenging activity with IC_(50) values of 0.168, 0.458 and 0.170 g·L~(-1), respectively. The DPPH radical scavenging rate of compound 1 was 41.1%.
Subject(s)
Free Radical Scavengers , Fruiting Bodies, Fungal , Chemistry , Ganoderma , Chemistry , Glycoside Hydrolase Inhibitors , Molecular StructureABSTRACT
Objective: To study the protective effect of the total flavones in Microcos paniculata (TFMP) on the acute myocardial ischemia (AMI) induced by isoprenaline (ISO) and its mechanism. Methods: The rats were ig administered with TFMP (8, 4, and 2 mg/kg) once daily for consecutive 5 d, and the AMI rat model was established by sc injection with ISO (2 mg/kg) 1 h after the last administration. The effects of TFMP on the electrocardiogram (ECG) at different time points and the myocardial tissue pathological histomorphology dying by hematoxylin were observed; The superoxide dismutase (SOD) and malondialdehyde (MDA) levels of myocardial tissue, glutathione peroxidase (GSH-Px), lactate dehydrogenase (LDH), and creatine kinase (CK) activities in serum were detected using biochemical method. Results: Compared with the model group, the high- and mid-doses (8 and 4 mg/kg) TFMP inhibited the J spot downward of ECG during myocardial ischemia (P < 0.05), especially after 10 min of ISO injection. The myocardial injury induced by ISO was aslo improved in these two groups, the levels of LDH and CK in serum (P < 0.05) and the content of MDA in myocardial homogenate (P < 0.01) were decreased, the activities of SOD (P < 0.01) and GSH-Px (P < 0.05, 0.001) in the myocardial homogenate were increased. Conclusion: The TFMP has the apparent protective effect on AMI injury, its mechanism may be related with improving the myocardial anti-oxidative ability and decreasing the oxidative stress reaction.
ABSTRACT
Objective To understand the synergistic effect of zinc and vitamin C on the anti-oxidative ability of sport training mouse. Methods 30 mice were randomly divided into 5 groups, blank control, normal saline solution, zinc , vitamin C and zinc + vitamin C. After 3 weeks of treatment, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the serum, liver and brain were determined. Results Compared with the control, the activity of SOD in the serum, liver and brain of the mice treated with zinc and vitamin C significantly increased, the content of MDA showed a significant decrease, the combination of zinc and vitamin C presented the best effect. Conclusion Combination of zinc and vitamin C has a significant synergistic effect in improvement of anti-oxidative ability.